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Siniša Mihajlovičius was dismissed from the position of head coach, and he will be replaced by Delio Rossi.
The club's leaders made this decision after Sunday's defeat against Verona's "Chievo" 0-1, after which Florence's team is only in 13th place in the standings.
"Mihajlovičius worked with all his heart, professionally, so we are extremely grateful to him and wish him success in the future," the statement from "Fiorentina" said.
Managed by a Serb, the team finished ninth in the "Serie A" championship in 2010-2011.
Meanwhile, Rossi, who coached the "Palermo" team last season, but was dismissed after another disagreement with the controversial president Maurizio Zamparini, signed a one and a half year contract.